We find striking evidence of strong economies of scale in crop breeding in China

The role and aim of food experiences is, therefore, not necessarily only to provide its customers with fresh produce but also to instill and create a sense of community for both the citizen as well as the consumer, who are no longer viewed as two separate categories. This historical division of consumer and citizen is rather questionable , and in recent literature the meanings of consumption, and thus use of things, are treated, rightly, with much seriousness as the full meanings and uses of objects to the consumer is more fully unfolded and understood. This is done even to the point where the object makes the human actors, involved with its transformation, its subjects. This can be seen in a historical perspective , or using present research methodologies of nonhuman agency . Event studies have, also, recently emerged as an original field of investigation separate from tourism and marketing, traditionally the most prominent areas of event research, although celebrations, events and exhibitions – in all their forms – have been used extensively within anthropology and sociology for multiple purposes. Today, there are entire dissertations focusing on food events, including analyses of different food scapes . The meanings of the “festive and/or affective food scape” in these last writings are multiple and their influence on both individuals and structures should not be underestimated in regards to community building and branding. These works show how certain food festivals thrive in some communities but not in others, and they document the continuing importance of food festivals, both internally and externally, for the hosting communities, as well as their branding of and economic potentials for the hosting city. Food events are legitimized by both social arguments of community building and economic arguments relating more to branding and economic benefits for participating cities. But it is not necessarily the events’ potentials to unite all these well-intended societal/economical intentions,maceteros reciclados de plastico be they based in community, communicative and economic wel-fare and/or reassuring reconciliation between the roles of the consumer and citizen; any surge in planned celebrations and events could also be a sign of what has been labelled a form of neo-localism.

A localism that – paradoxically – might have arisen due to the removal of spatial barriers and the emergence of globalised society: “The elaboration of place-bound identities has become more rather than less important” . Place, and its celebration is, therefore, pursued, valued and marketed more, exactly because its significance, in the traditional sense, is actually vanishing. When you can go anywhere, you want to be somewhere, and somewhere that is perceived as “real.” It should be noted that this form of boosterism using events is not a new phenomenon. In an American context agricultural celebrations and fairs have been used extensively to promote rural places . These events even serving as “feeders” of exhibition materials and activities to the World Fairs, which, arguably, together with the Olympics, have been the events to host for any international metropolis, or those seeking to become one, through the late nineteenth century and most of the twentieth century, and to which we will return. There are several explanations and theories as to the rise of the importance of experiences in most developed countries. Pine and Gilmore mention the structural development that people, in general, have accumulated more expendable income to spend on experiences overall. Experiences and events can therefore be seen as the transitory symptom/symbol of moving from an industrial society to a knowledge/service society. This development of an experience economy is also linked to the commercialization of traditions and “glocalization” of culture coupled with a general broadening in people’s cultural orientation, where individuals are not necessarily limited to one way of life, but can throughout their lives experience and/or consume many different lifestyles. It is sometimes suggested that social interaction and related experiences are on the decline in contemporary society . The bedevilled individualization is often accused of being the main perpetrator of this decline, though studies suggest that neither sociability nor its associated experiences and events are under threat. A larger study by Ingen & Dekker makes the case that many perceptions held about the decline of traditional community pursuits and celebrations is not necessarily due to less sociability, but can be explained, rather, by an increase in informalization processes.

This means that traditional social activities, be they through formal memberships in clubs, associations or otherwise, might be replaced, or supplemented, by more informal settings and occasions that do not require memberships or particular spatial environments, for instance. The decline in – say – bowling memberships can thus be offset by other social interaction less recordable, but not necessarily less sociable. This process of informalization could be viewed as part of a larger social “re-arrangement” where individuals partaking in the “Network Society” find new outlets and forms to interact and express sociability: “Knowledge transfer takes place within defined circuits between different groups and ‘scenes’ in the creative sector. One of the essential requirements of this system is physical spaces where people can meet and validate new cultural forms, or ‘play-grounds of creativity’ such as cafes, squares, museum foyers. These are also the new spaces that are often so attractive to tourists” .Castell’s, himself, is an advocate of networks to further sociability for the individual – though admittedly these expressions will be mostly utilized by already well-connected people, and critical voices are heard as to the nature of such “networking individuals and their sociability”: “The mobility and independence of network nomads who swing from contact to contact and project to project, socially and spatially, without insisting on a consistent self image, is now considered the most valuable asset of human capital” . Increased individualization, or individual freedom, might actually lead to more sociability as expressed through events and other informal spatial arrangements. These might differ slightly or significantly from the form and content of their predecessors of yesteryears, but their use as vehicles of expression and meaning to community and beyond surely remains – though, admittedly, community and associated meanings might also be ontologically different than yesteryears’, if we agree, that we are moving from industrial to knowledge society in the developed world. Urbanization has become coupled with the legitimization of new economic instruments and parameters and their importance, where events and cultural symbols play a determining role, as metropolises compete globally for attention and money through culture-economic initiatives, while midsized cities compete regionally, and so on.

The particular frequency and intensity of urban competition and development might be particular to contemporary society, but using events and experiences to further urbanization is not new. The Olympic Games, for instance, have been used extensively as a “catalyst of urban change” throughout the twentieth century: “What began simply as a festival of sport has grown into an unusually conspicuous element in urban global competition and, for its host cities, a unique opportunity to attract publicity, bring in investment and modernize their infrastructures and images” . And it might not just be commercialization of traditions taking place but also traditionalization, or “culturalization,” of commerce or the mix of worldly and spiritual spheres. Marxists might interpret this next step as the ultimate alienation of man and nature secured through an advanced form of commodity fetishism as exemplified by Debord’s “Spectacle Society” , but its materiality and presence in everyday life is undeniable, and perhaps most illustratively performed at the celebrations and events of our times. Could such developments be due to the merger between citizen and consumer? The blurred lines between citizen/consumer might work to influence both market and non-market,fabrica de macetas plasticas as the market is attached to traditional non-market values and vice versa as is evident in the rise of farmers markets, food co-ops, for instance. It should be noted, however, that the strong division between that of the consumer and citizen could be somewhat of an historical illusion, as the two categories have always transgressed and shared common areas . Again such sharp distinctions could be due to an idealization of the past, whose societies so often are narrated as less complex than present society. Food, and to some degree agriculture, and its associated experiences, carry much significance today, as symbols of the longevity, and dare one say the sustainability of societies and communities in general, and as media and/or vehicles for social change, as well as a symbolic and very real mark of distinction and identity. These observations are in themselves nothing new, as previous literature has proficiently shown that food and its associated experiences have been used in multiple ways . But the particular form and associated expressions and meanings associated with these might be, somewhat, unique to our present times. It is hard not to recognise the influence of the “risk society” in the above themes and the themes they invoke, along, or together, with the perceived negative separation between man and nature that is reminiscent of earlier literature also involving the urban/rural continuum, which suggests real differences between urbanity and rurality , dismisses these differences , or suggests that the urban/rural continuum continues to hold real significance as part of an identification of special rural and urban qualities .

The rural/urban continuum illustrates, perhaps better than anything, the condition of post modernity where “imagined” and/or perceived differences are as important as any “real” measurable differences – indeed reality is guided by perceptions of the “other” and associated places. Due to increased mobility, place should, in theory, matter less, but this does not seem to be the case. The importance of place, and its celebration, can be explained by increasing globalization and homogenization – including cultural commodification and standardization – where global structures are often associated with homogenizing markets and local structures associated with diverse community values, the latter often being perceived as intrinsically better exactly because of its locality or adherence to place. Though literature of historic consumption provides ample examples of how global trade interacts and changes the local , and that viewing the local as a separate entity from the global might be counterproductive and/or naïve. The strongly felt presence of concepts such as authenticity, could be explained due to the, perceived, alienation between man and nature/agriculture generating farmers markets, school gardens etc. to cure ills and provide solutions that have barely been articulated before like eco-literacy or food literacy. Authenticity can, also, provide individuals living under the, perceived, fragmented and illusive condition of post-modernism a re-assurance of their future choices by invoking ties to nature and community, which are seen as good due to their perceived universal/stable/traditional structures. Again this complexity, and the acknowledgement of its existence, can actually strengthen the desire for authenticity. Authenticity within food consumption and places can thus be viewed as an elitist concept that through the appropriation of communal spaces and individual consumption patterns makes opaque the ‘actual’ societal and political structures that guide our food consumption and understanding. But also it could be seen as the first step toward a new way of thinking about food and social systems and food scapes that dares to question the productivist rationale of modernism and its spatial expressions, invoking and awakening a new understanding of, and relation between, the local and global – probably it will do both. This conceptual sentiment of doing both is perhaps the “true” lesson of post-modernism, if we accept its premise. Concepts like authenticity have the thematic potentials to address conflicting messages and provide contradictory explanations to societal issues. Maybe this ability is also mirrored in our materiality of the twenty-first century and the growing acknowledgement that we can produce more of everything for everybody and still – even if it is “only” on a perceptual level – miss that something of the past, imagined or otherwise. The increased individualization might run parallel to an increased socialization. But both are different forms and hold different aspects and experiences than individuality and sociability thirty, or even a hundred years ago. Providing credence to the concept of a universal humanism that is shared, but also one that is provided new meanings and forms by its participants, both good and bad, throughout history. Indeed, and this is the paradox, the mastery and control of nature which is the basis of our modern food systems can be perceived as alienating and unnerving.

The overall synthesis of the trehalose hydrogel was modified from a previously reported procedure

Multi-substituted styrenyl-trehalose prepared during the monomer synthesis acted as an efficient cross-linker to form a hydrogel after redoxinitiation with APS/TEMED , eliminating the need for additional cross-linkers or chromatographic purification of the monomer. After formation, the crude hydrogel was extensively washed via Soxhlet extraction in order to remove any unreacted trehalose and other residual non-polymerized components in the precipitation mixture, producing a colorless gel. Finally, the gel was lyophilized to produce a white powder. Originally, this method only produced hydrogel with an overall low yield of 17 %,which is too low to be relevant for use. Thus, we systematically optimized for the hydrogel synthesis to increase scale and sustainability. To increase the yield, we decreased the equivalents of trehalose from 5 to 3.33, effectively increasing the ratio of 4-vinylbenzyl chloride to trehalose. LC/MS of the dried solids confirmed that the product contained a mixture of mono- and di-substituted styrenyl-trehalose with various regioisomers at 94.1 % and 5.9 %, respectively . Higher order components such as tri-substituted trehalose were not observed by LC/MS either because they too dilute to detect or they were not ionizable. We then degassed the monomer/cross-linker and initiator solutions prior to gelation. This gave an overall reaction yield of 88 %. The increase in 4-vinylbenzyl chloride allowed for an increase in modified trehalose in the crude trehalose monomer/cross-linker mixture , which in turn resulted in a higher yield. When this improved synthesis was scaled 100-fold, it was carefully monitored with regard to reaction conversion, solvent removal, and drying to ensure that trehalose was sufficiently modified for gelation. With these necessary modifications, such as slow precipitation, the multi-gram reaction gave an overall yield of 75.6 %. As the goal for reaction yields in industry is above 70 %, this yield is industrially-promising.The final product demonstrated a storage modulus that was larger than the loss modulus as determined by a rheology frequency sweep with constant strain,maceta de plastico cuadrada indicating that the material passed its gel point and demonstrated solid-like behavior.

The swelling ratio was calculated as 15.63 ± 0.71 indicates that the material absorbs water many times its dry weight, as is characteristic of hydrogels.Though both a high yield and scalable synthesis were demonstrated, there are other factors that are desirable for industrial use, such as minimal use of hazardous materials, especially halogenated and toxic solvents.The precipitation step to isolate the crude reaction mixture was originally undertaken in hexanes, a neurotoxin,and DCM, a carcinogen.To replace these solvents, we tried to eliminate the precipitation step. This attempt was irreproducible and therefore not explored further. We next endeavored to replace hexanes and DCM with sustainable solvents of similar polarities. Based on waste, environmental, health, and safety issues, GlaxoSmithKline has produced a guide which scores solvents on sustainability.The solvents are assigned colors, red, amber, or green, where red is reserved for problematic solvents, such as hexanes and DCM, green is for the more sustainable solvents, and amber is for solvents in between the two extremes. Heptane and toluene are amber solvents with similar polarities as hexanes and were therefore envisioned as substitutes for hexanes. Acetone, an amber solvent, butanone, a green solvent, and ethyl acetate, a green solvent, with higher polarity, were explored as substitutes for DCM. Solvent ratios were varied to ensure miscibility of DMSO. Acetone, ethyl acetate, and butanone were too polar to sufficiently precipitate modified trehalose. Yet, when combined with heptane or toluene, gels formed after the precipitation step . The overall yields of these gels were lower because of the employed washing method. Material is lost when decanting and replacing solvent versus Soxhlet extraction; this washing method was necessary due to the small scale of the reactions.Precipitating into green/amber solvents gave similar yields , with the exception of butanone/heptane . The most sustainable precipitation that gave the highest yield, ethyl acetate/toluene , was scaled 100-fold and then used for gelation to give a 64.1 % yield. This yield can likely be optimized further with industrial equipment and shows potential as a greener alternative to the original synthesis.

It is important to note that although 4-vinylbenzyl chloride exhibits acute toxicity, we previously demonstrated that once reacted to form styrene-modified linear trehalose polymers, the material is cytocompatible and similar styrenyl polymers are nontoxic in vivo. 23With an optimized synthesis, the gels were tested for their efficacy in protecting animal feed enzymes during the conditions encountered in the feed pelleting process. Steam pelleting is a common practice as it improves feed efficiency by decreasing nutrient degradation.Frequently, temperatures reach 60 °C to 90 °C and most enzyme activity is lost at such temperatures. A method to stabilize these enzymes during pelleting that offers a dry, easy-to-handle final form of feed with active enzyme is essential to the livestock industry. We decided to investigate phytase, xylanase, and b-glucanase because 60 % of the global feed enzyme market is attributed to phytase, while 80 % of the global carbohydrase market is xylanase and b-glucanase.Phytase hydrolyzes phytate, which is the storage form of phosphorus in the feed, releasing digestible phosphates and chelated minerals.Xylanase degrades the main component of hemicellulose present in plant cell walls, b- 1,4-D-xylan, into xylose.b-Glucanase cleaves 1,4-b-D-glycosidic linkages in b–glucan, allowing the release of smaller saccharides from cellulose.For xylanase, the activity beyond 100 % retention invoked by the addition of trehalose hydrogel to phytase and xylanase may be attributed to the gel scaffold or trehalose moieties stabilizing or enhancing the protein/substrate binding and/or stabilizing the protein to the assay conditions that includes a lyophilization step. The former has been observed for glucose oxidase where the addition of trehalose augmented the affinity of the substrate for the enzyme,36 and for enzymes horseradish peroxidase and b-galactosidase upon interaction with trehalose polymer excipients.We investigated the activity of xylanase after incubation with hydrogels, without heat. Indeed, xylanase activity was greater than 100 % by the addition of even 1 weight equivalents of trehalose hydrogel .

Given that trehalose hydrogel alone has negligible signal from the enzyme assays, these results suggest that the trehalose hydrogel could be stabilizing the enzyme even without the addition of heat. The heated xylanase data was normalized to the results in Figure 2.7, and trehalose hydrogel still demonstrated substantial stabilization of the enzyme . We then considered the activity of xylanase when incubated with free trehalose without heat. We observed greater than 100 % original activity, albeit with no statistical difference compared to xylanase activity without additive . It follows that the gel scaffold or multi-valent effect of polymerized trehalose, beyond that of free trehalose, could be responsible for the improved activity of xylanase when incubated with hydrogel. We investigated the increase in activity of xylanase further by testing the aggregation state of xylanase subjected to heated assay conditions with or without hydrogel additives and comparing the results to a control subjected to all the assay conditions except for the heat step. An additional sample of fresh xylanase was prepared. At 1 mg/mL xylanase concentration in 0.1 M sodium acetate, all conditions were evaluated by dynamic light scattering . The results demonstrated small hydrodynamic sizes for fresh xylanase as well as xylanase subjected to heat with hydrogel additive. However, large increases in hydrodynamic size , indicative of aggregate formation, were observed for free xylanase subjected to assay conditions with or without heat. Thus,maceteros reciclados de plastico free xylanase must be sensitive to assay conditions, which includes a lyophilization step, and hydrogel addition stabilizes xylanase to these conditions, thereby likely explaining the greater activity. Trehalose polymers stabilize proteins to both lyophilization and heat by suppressing water crystallization, preventing aggregation and helping to refold proteins,and likely the hydrogels act by similar mechanisms. The release of enzymes within the gel was tested. Phytase was chosen as the model enzyme as it has the highest molecular weight compared to xylanase and b-glucanase and should therefore have the slowest diffusion rate of the three enzymes. The experiment was conducted at pH 6.3 using simulated intestinal fluid,37 and 37 °C to mimic the conditions found within the small intestine of pigs.FITC-labeled phytase demonstrated 100 % release from the trehalose hydrogels within 4 hours . It has been estimated that transit of feed through the small intestines of pigs is approximately 3 – 4 hours.Thus, the release is already within the relevant time frame and will likely be expedited with increased agitation in the gastrointestinal tract. We also demonstrated that the addition of trehalase enzyme had no effect on the release of phytase from the trehalose hydrogels . Trehalase is an enzyme found in the intestines and kidneys of many organisms, including humans and pigs,that catalyzes the conversion of trehalose into two glucose units. Therefore, we hypothesized that trehalase could potentially breakdown trehalose within the hydrogel, catalyzing its degradation and release of sequestered cargo. However, previous studies demonstrate that disaccharide mimics are completely buried within the trehalase enzyme, requiring significant conformational changes for substrate entry into the enzyme’s active site.Thus, it is likely that the interaction between trehalose linked within the hydrogel and trehalase’s active site is entropically unfavorable or sterically forbidden, preventing degradation. Note that the increase in trehalase concentration from 3.5 U/mL to 1000 U/mL in this experiment still had no effect on phytase release .Lastly, we explored whether trehalose polymers could similarly stabilize whole cells.

A linear trehalose glycopolymer with the same backbone as the trehalose hydrogel was evaluated for its ability to stabilize bacteria to lyophilization, which is often used to preserve and store biological samples, but is also known to negatively affect protein and cell viability .The trehalose glycopolymer was added as an excipient to a strain of BL21 E. Coli bacteria under lyophilization stress. Cell proliferation was monitored by measuring the OD600 and was used to determine E. coli survival and, therefore, stabilization. Samples lyophilized in the presence of P3 had lower OD600 measurements compared to with trehalose at an equivalent concentration , but higher OD600 measurements compared to the control . While trehalose has been shown to stabilize cells in a the dry state by maintaining the structural and functional integrity of their membranes through vitrification and bacteria synthesize extracellular polysaccharides in preparation for dehydration,cytosolic trehalose has been shown to be more effective for cell stabilization than extracellular trehalose added as an excipient.As such, it may be necessary to internalize trehalose, as the free disaccharide or the glycopolymer, within the cell to see more significant stabilization. All reagents and solvents were purchased from SigmaAldrich or Fisher Scientific and used without further purification unless otherwise noted. Trehalose was purchased from The Endowment for Medical Research and was azeotropically dried with ethanol and kept under vacuum until use. Phytase was provided by Phytex, LLC. Xylanase and trehalase werepurchased from Megazyme. b-glucanase was purchased from Sigma-Aldrich. EnzChek Ultra Xylanase Assay Kit was purchased from Fisher Scientific. b-Glucanase Assay Kit was purchased from Megazyme. Kanamycin-resistant strain of BL21 E. Coli bacteria was provided by the lab of Professor Robert Clubb. 1 H-NMR spectra were performed on an Avance DRX 400 MHz instrument. Liquid chromatography-mass spectrometry experiments were carried out on an Agilent 6350 QTOF ESI with a 1260 Infinity LC and Phenomenex Luna C18 5 µm column and were eluted with a gradient of 5 – 95% solvent B over 20 minutes. To determine hydrogel mechanical properties, an AR 2000ex rheometer in parallel plate geometry was used with an 8-mm diameter stainless steel, cross hatched upper plate and 60 mm stainless steel, cross hatched lower plate cover, at 22 °C, constant strain of 1%, and an angular frequency range of 0.1 to 10 rad s-1 . Gels were swelled for 72 hours in Milli Q water, trimmed to 8 mm diameter and approximately 1 mm thickness, and carefully blotted to removed excess water before measurements. Absorbance measurements to determine the degree of fluorescein isothiocyanate labeling of phytase and concentration of protein were conducted on a Thermo Fisher Scientific NanoDrop 2000 Spectrophotometer. Other absorbance and fluorescence measurements were taken on a Tecan Infinite M1000 plate reader. Dynamic light scattering measurements were conducted on a Malvern ZetaSizer Nano at 1 mg mL-1 protein concentration in 0.1M sodium acetate buffer.Under an inert atmosphere of argon gas, sodium hydroxide pellets were added followed by dimethylsulfoxide .